Bisphenol a influences blastocyst implantation via regulating integrin beta 3 and trophinin expression levels

作者：Pan, Xiaoyan; Wang, Xiyan; Wang, Zhengchao; Wang, Xuenan; Dou, Zhaohua; Li, Zhixin^*

来源：International Journal of Clinical and Experimental Medicine, 2015, 8(11): 20035-20045.

• Blastocyst implantation
• bisphenol A (BPA)
• integrin beta 3
• trophinin
• estrogen receptor

Objective: This study is to investigate effects of bisphenol A (BPA) on the blastocyst implantation in endometrium. Methods: Pregnant mice were orally administered with BPA. Implantation sites were examined, and serum estrogen level was assayed with ELISA. Protein expression levels were detected with immunohistochemistry and immunofluorescence staining. Results: High doses (400 and 600 mg/kg/day) of BPA remarkably reduced the implantation sites in the pregnant mice. No significant differences were observed in the serum estrogen level across the groups. Moreover, high doses (400 and 600 mg/kg/day) of BPA significantly declined the expression level of endometrial estrogen receptor a (ERa) in the pregnant mice. In addition, high doses (400 and 600 mg/kg/day) of BPA significantly declined the expression levels of integrin beta 3 and trophinin in the endometrium and blastocysts. Conclusion: BPA declines ERa expression in endometrium, and inhibits adhesion protein expression in endometrium and blastocysts, causing the adhesion failure of blastocyst implantation.

• 出版日期2015
• 单位福建师范大学; 济宁医学院