Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay

作者：Celina Bonetto M; Sacco Natalia J; Hilding Ohlsson Astrid; Corton Eduardo

来源：Talanta, 2011, 85(1): 455-462.

DOI：10.1016/j.talanta.2011.04.007

• Amperometric
• Bioassay
• BODSEED
• Ferricyanide
• Formaldehyde
• Klebsiella pneumoniae

Methods for short-term BUD analysis (BOD(st)) based on ferricyanide mediator reduction have succeeded in overcoming some problems associated with the standard BUD test analysis (BOD(5)) such as long-term incubations (5 days), the need to dilute samples and low reproducibility. Here we present a bioassay where a Klebsiella pneumoniae environmental strain successfully reduces ferricyanide without de-aeration of the samples with linear BOD(5) ranges between 30 and 500 mg L(-1) or 30 and 200 mg L(-1), using glucose-glutamic acid solution (GGA) or OECD standards respectively. We further propose a new assay termination solution that allows higher reproducibility and standardization of the cell-based assay, employing formaldehyde (22.7 g L(-1)) or other compounds in order to stop ferricyanide reduction without affecting the amperometric detection and therefore replace the centrifugation step normally used to stop microbial-driven reactions in ferricyanide-mediated bioassays. These improvements led to an accurate determination of real municipal wastewater samples.

• 出版日期2011-7-15