Novel sensitive, specific and rapid pharmacogenomic test for the prediction of abacavir hypersensitivity reaction: HLA-B*57:01 detection by real-time PCR

作者：Dello Russo Cinzia^*; Lisi Lucia; Lofaro Alessia; Di Giambenedetto Simona; Federico Bruno; madeddu Giordano; Salerno Marianna; Mura Maria Stella; Pirazzoli Antonella; de Luca Andrea; Cauda Roberto; Navarra Pierluigi

来源：Pharmacogenomics, 2011, 12(4): 567-576.

DOI：10.2217/PGS.10.208

• abacavir
• HIV-1
• HLA-B*57:01
• hypersensitivity reaction
• pharmacogenomic test
• real-time PCR

Aim: International HIV treatment guidelines recommend HLA-B*57:01 typing before abacavir administration, in order to reduce the incidence of abacavir hypersensitivity reactions, the major cause of early therapy discontinuation. A fast, sensitive and specific test for HLA-B*57:01 detection has been developed in the present study. Materials & methods: Two sets of sequence-specific primers were designed, and amplification rapidly detected by real-time PCR. Results: A total of 108 samples were analyzed in a single-blind fashion, and 41 samples were identified as positive. Complete agreement, with kappa = 1 (standard error = 0.0962, p < 0.0001), was found, with a validated methodology used in the EPI109367 clinical trial funded by GlaxoSmithKline, and consisting of low-resolution sequence-specific oligonucleotide PCR, followed by high-resolution sequence-specific oligonucleotide PCR carried out on the HLA-B*57-positive samples. Conclusion: We provided a detailed characterization of a novel HLA-B*57:01 screening test, which can be easily implemented by those laboratories already involved in the detection of viral load and virus genotyping.

• 出版日期2011-4