Large-scale image-based screening and profiling of cellular phenotypes

作者：Bougen Zhukov Nicola; Loh Sheng Yang; Lee Hwee Kuan; Loo Lit Hsin^*

来源：Cytometry, Part A, 2017, 91A(2): 115-125.

DOI：10.1002/cyto.a.22909

• high-content screening
• phenotypic profiling
• imaging-based phenotypic screens
• automated image analysis
• cellular phenotypes
• high-throughput microscopy

Cellular phenotypes are observable characteristics of cells resulting from the interactions of intrinsic and extrinsic chemical or biochemical factors. Image-based phenotypic screens under large numbers of basal or perturbed conditions can be used to study the influences of these factors on cellular phenotypes. Hundreds to thousands of phenotypic descriptors can also be quantified from the images of cells under each of these experimental conditions. Therefore, huge amounts of data can be generated, and the analysis of these data has become a major bottleneck in large-scale phenotypic screens. Here, we review current experimental and computational methods for large-scale image-based phenotypic screens. Our focus is on phenotypic profiling, a computational procedure for constructing quantitative and compact representations of cellular phenotypes based on the images collected in these screens.

• 出版日期2017