Acetylcholine Esterase Inhibitory Potential and Antioxidant Activity of Various Extracts of Leucas cephalotes and Juglans regia L.

作者：Shahwar Durre^*; Naz Mi**ah; Raza Muhammad Asam; Ara Gulshan; Yasmeen Asma; Saeed Afifa; Bokhari Sana; Ajaib Muhammad; Ahmad Naeem

来源：Asian Journal of Chemistry, 2012, 24(7): 3151-3154.

• Acetylcholine esterase
• Antioxidant activities
• Leucas cephalotes
• Juglans regia L.

The aerial parts of Leucas cephalotes were extracted in n-hexane, chloroform and ethyl acetate successively, while seeds of Juglans regia L. were extracted in n-hexane and ethyl acetate. Column chromatography of the n-hexane fraction of J. regia yielded eight fractions. Total phenolic contents were calculated using Folin-Ciocalteu (FC) reagents while, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging, ferric reducing antioxidant power (FRAP) and acetylcholine esterase (AChE) inhibition potential of all extracts/fraction was evaluated according to their respective in-vitro models. Ethyl acetate extract of L. cephalotes contained highest total phenols (864.5 +/- 1.2 mg GAE/g of extract) with 83.3 +/- 1.1 % DPPH activity and IC50 value 271 +/- 6 mu g/mL. The column fractions of J. regia (JRH-8) demonstrated significant antiradical activity (80.8 +/- 1.1 %, IC50 = 283 +/- 4 mu g/mL). All extracts/fractions showed remarkable FRAP activity. The n-hexane extracts of both plants exhibited maximum enzyme inhibition activity 78.7 +/- 1.1 % (IC50 = 175 +/- 4 mu g) and 73.5 +/- 1.5 % (IC50 = 108 +/- 3 mu g) for J. regia and L. cephalotes respectively.

• 出版日期2012-7