Macrocalyxin A inhibits proliferation and induces apoptosis of t (8;21) leukemia cells through mitochondrial signaling pathways and regulates AML-ETO mRNA expression

作者：Wang, Zhenni; Lv, Yaping; Xia, Jun; Shi, Hao; Wang, Weizhong; Zhou, Yonglie^*

来源：Molecular Medicine Reports, 2015, 12(3): 3537-3542.

DOI：10.3892/mmr.2015.3855

• macrocalyxin A
• kasumi-1 cell
• apoptosis
• AML-ETO

Progress in the last decade has improved the treatment of acute myeloid leukemia (AML); however, the treatment of AML is also demanding and better treatments are required. The present study aimed to examine the antiproliferative and proapoptotic effects of macrocalyxin A (MA), a novel deterpenid compound, on AML cells. It was identified that MA significantly inhibits kasumi-1 cell proliferation in a time-and dose-dependent manner. Furthermore, low concentrations of MA were able to induce kasumi-1 cell differentiation; however, high concentrations of MA induced kasumi-1 cell apoptosis. MA was also able to increase the expression of mitochondrial membrane protein in a dose-dependent manner while the Delta Psi m was reduced. Additionally, Bad expression in kasumi-1 cells was increased when treated with MA, indicating that the intrinsic apoptotic pathway may be important in MA-induced kasumi-1 cell apoptosis, where the mitochondrial permeability transition pore is opened and the Delta Psi m is reduced. In addition, it was demonstrated that AML-ETO mRNA may also be important in MA-induced apoptosis.

• 出版日期2015-9
• 单位浙江省人民医院; 浙江工业大学