In vivo comet assay of multi-walled carbon nanotubes using lung cells of rats intratracheally instilled

作者：Ema Makoto^*; Masumori Shoji; Kobayashi Norihiro; Naya Masato; Endoh Shigehisa; Maru Junko; Hosoi Masayo; Uno Fuyumi; Nakajima Madoka; Hayashi Makoto; Nakanishi Junko

来源：Journal of Applied Toxicology, 2013, 33(10): 1053-1060.

DOI：10.1002/jat.2810

• nanomaterials
• multi-walled carbon nanotubes
• genotoxicity
• comet assay
• lung
• intratracheal instillation
• inflammation

The genotoxicity of multi-walled carbon nanotubes (MWCNTs) was evaluated in vivo with comet assays using the lung cells of rats given MWCNTs. The MWCNTs were intratracheally instilled as a single dose at 0.2 or 1.0 mg kg(-1) or a repeated dose at 0.04 or 0.2 mg kg(-1), once a week for 5 weeks, to male rats. The rats were sacrificed 3 or 24 h after the single instillation and were sacrificed 3 h after the last instillation in the repeated instillation groups. Histopathological examinations of the lungs revealed that MWCNTs caused inflammatory changes including the infiltration of macrophages and neutrophils after a single instillation and repeated instillation at both doses. In comet assays using rat lung cells, no changes in % Tail DNA were found in any group given MWCNTs. These findings indicate that MWCNTs do not have the potential to cause DNA damage in comet assays using the lung cells of rats given MWCNTs at doses causing inflammatory responses.

• 出版日期2013-10