A comprehensive characterisation of beer polyphenols by high resolution mass spectrometry (LC-ESI-LTQ-Orbitrap-MS)

作者：Quifer Rada Paola; Vallverdu Queralt Anna; Martinez Huelamo Miriam; Chiva Blanch Gemma; Jauregui Olga; Estruch Ramon; Lamuela Raventos Rosa^*

来源：Food Chemistry, 2015, 169: 336-343.

DOI：10.1016/j.foodchem.2014.07.154

• Beer
• Polyphenols
• LTQ-Orbitrap
• Phenolic acids
• High resolution mass spectrometry

Beer is the second most consumed alcoholic beverage in Europe and shown by the European Prospective Investigation into Cancer and Nutrition cohort study to be the main food contributor to hydroxybenzoic acid intake. About 70-80% of the total polyphenol content in beer comes from malt, and the remaining 30-20% from hops. In this work, liquid chromatography coupled with an electrospray ionization hybrid linear ion trap quadrupole Orbitrap mass spectrometry technique has been used for an accurate identification of beer polyphenols. 47 phenolic compounds were identified using high mass accuracy and confirmed by MS2 experiments, including simple phenolic acids, hydroxycinnamoylquinics, flavanols, flavonols, flavones, allcylmethoxyphenols, alpha- and iso-alpha-acids, hydroxyphenylacetic acids and prenylflavonoids. As far as we know, 7 of these compounds have been recognised in beer for the first time: feruloylquinic acid, caffeic acid-O-hexoside, coumaric acid-O-hexoside, sinapic acid-O-hexoside, catechin-O-dihexoside, kaempferol-0-hexoside, and apigenin-C-hexoside-pentoside.

• 出版日期2015-2-15