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摘要
To survive at the body temperature of their hosts, pathogenic fungi have developed important molecular mechanisms, such as protein expression associated with growth at high temperatures. Thus, the aim of this study was to analyze the in vitro growth of Conidiobolus lamprauges at different temperatures and compare proteins expressed by two-dimensional electrophoresis (2D), for the pathogen cultivated at low (28 degrees C) and high (37 degrees C) temperatures. For the analysis of growth temperatures, five isolates of C. lamprauges from sick sheep were incubated at 20, 25, 30, 35 and 40 degrees C and radial growth was measured every 24 hours. For the analysis of differential expression, protein extraction and two-dimensional polyacrylamide gel electrophoresis were performed with C. lamprauges cultivated at 28 degrees C and 37 degrees C for 48 hours. The average radial growth was different at the temperatures tested, and 35 degrees C was found to be the best growth temperature for all isolates. The optimum adjusted temperature ranged between 33.3 degrees C and 34.8 degrees C. The upper and lower limits of growth inhibition were 18 degrees C and 42 degrees C, respectively. Upon expression analysis, a total of 16 spots were differentially expressed, seven (7/16) proteins were downregulated and nine (9/16) were over-expressed at 37 degrees C compared to 28 degrees C. In addition, eight spots were present only at 28 degrees C and six were present only at 37 degrees C. It is suggested that C. lamprauges produces a profile of proteins that is related to thermoregulation triggered by the high temperature of the host.
- 出版日期2014-3
