Nanosensor dosimetry of mouse blood proteins after exposure to ionizing radiation

作者:Kim Dokyoon; Marchetti Francesco; Chen Zuxiong; Zaric Sasa; Wilson Robert J; Hall Drew A; Gaster Richard S; Lee Jung Rok; Wang Junyi; Osterfeld Sebastian J; Yu Heng; White Robert M; Blakely William F; Peterson Leif E; Bhatnagar Sandhya; Mannion Brandon; Tseng Serena; Roth Kristen; Coleman Matthew; Snijders Antoine M; Wyrobek Andrew J*; Wang Shan X
来源:Scientific Reports, 2013, 3(1): 2234.
DOI:10.1038/srep02234

摘要

Giant magnetoresistive (GMR) nanosensors provide a novel approach for measuring protein concentrations in blood for medical diagnosis. Using an in vivo mouse radiation model, we developed protocols for measuring Flt3 ligand (Flt3lg) and serum amyloid A1 (Saa1) in small amounts of blood collected during the first week after X-ray exposures of sham, 0.1, 1, 2, 3, or 6 Gy. Flt3lg concentrations showed excellent dose discrimination at %26gt;= 1 Gy in the time window of 1 to 7 days after exposure except 1 Gy at day 7. Saa1 dose response was limited to the first two days after exposure. A multiplex assay with both proteins showed improved dose classification accuracy. Our magneto-nanosensor assay demonstrates the dose and time responses, low-dose sensitivity, small volume requirements, and rapid speed that have important advantages in radiation triage biodosimetry.

  • 出版日期2013-7-19