MicroRNA-133b inhibits cell proliferation and promotes apoptosis by targeting cullin 4B in esophageal squamous cell carcinoma

作者：Huang, Hairong; Xu, Yang; Guo, Zhong; Chen, Xi; Ji, Shaiguang; Xu, Zhiyun^*

来源：Experimental and Therapeutic Medicine, 2018, 15(4): 3743-3750.

DOI：10.3892/etm.2018.5906

• microRNA-133b
• esophageal squamous cell carcinoma
• proliferation
• apoptosis
• cullin 4B

microRNAs (miRs) serve a role as modulators during carcinogenesis. It has been demonstrated that the expression of miR-133b is decreased in a variety of tumor tissues and cell lines and serves a suppressive role in the proliferation and apoptosis of different types of tumor cells. However, its effect on esophageal squamous cell carcinoma (ESCC) cells remains unclear. In the current study, the expression of mature miR-133b was measured using reverse transcription-quantitative polymerase chain reaction and the results indicated that miR-133b was significantly downregulated in ESCC tissues and various ESCC cell lines. The overexpression of miR-133b significantly inhibited the proliferation and promoted the apoptosis of KYSE150 and Eca-109 cells. Furthermore, it was demonstrated that cullin 4B (CUL4B) promotes ESCC cell proliferation and inhibits apoptosis by activating the protein kinase B/glycogen synthase kinase 3/-catenin pathway. Taken together, these results demonstrate that miR-133b/CUL4B serves a tumor suppressive role during ESCC progression and may therefore be used as a potential target to treat patients with ESCC.

• 出版日期2018-4
• 单位中国人民解放军第二军医大学; 南京大学