Molecular cloning and sequence of gilthead sea bream (Sparus aurata) alpha-skeletal actin: tissue and developmental expression

作者：Moutou KA^*; Socorro S; Power DM; Mamuris Z; Canario **M

来源：Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, 2001, 130(1): 13-21.

DOI：10.1016/S1096-4959(01)00381-5

• alpha-actin cDNA
• gilthead sea bream (Sparus aurata)
• skeletal muscle
• teleost

In the present study, the Sparus aurata alpha -skeletal actin was cloned from a mixed larvae complementary DNA library. The clone isolated was 1523 bp long with an open reading frame of 1134 bp coding for a 377-amino acid protein. The deduced amino acid sequence of sea bream a-actin is identical to Fugu alpha -actin-1. The expression of a-actin was initiated at the onset of segmentation. In adult fish, alpha -actin is expressed predominantly in white and red muscle.

• 出版日期2001-8