Acute phase response in experimentally Escherichia coli serotype O55:B5 induced endotoxemia and its comparative treatment with dexamethasone and flunixin meglumine in Iranian fat-tailed sheep

作者：Chalmeh Aliasghar^*; Badiei Khalil; Pourjafar Mehrdad; Nazifi Saeed

来源：Veterinarski Arhiv, 2013, 83(3): 301-312.

• endotoxemia
• acute phase response
• dexamethasone
• flunixin meglumine
• Iranian fat-tailed sheep

This experiment was performed in order to clarify the effect of acute phase response following the induction of endotoxemia by Escherichia coli serotype O55:B5 and regarding its comparative treatment with dexamethasone and flunixin meglumine in the Iranian fat-tailed sheep. Fifteen, clinically healthy, one-year old Iranian fat-tailed ewes were randomly assigned into three equal (n = 5) experimental groups, comprising of a control group, and a Dexa and a Flnx group. Lipopolysaccharide from Escherichia coli serotype O55:B5 was administered intravenously at 20 mu g/kg. All experimental groups underwent intravenous fluid therapy for 120 minutes after lipopolysaccharide injection. 180 minutes after lipopolysaccharide, Dexamethasone (in Dexa group at 1 mg/kg) and flunixin meglumine (in Flnx group at 2.2 mg/kg) were injected, along with the intravenous fluid for 60 minutes. The control group received only lipopolysaccharide and was treated with an intravenous fluid without any drug. The researchers collected blood samples from all the ewes and assayed separated sera for serum acute phase proteins (serum amyloid A and haptoglobin) and inflammatory cytokines (tumor necrosis factor-alpha and interferon-gamma). In all experimental groups there a rapid increase was noted in the amount of acute phase proteins and inflammatory cytokines after endotoxemia induction (P%26lt;0.05). At the same time, there were no significant differences in the amount of acute phase proteins and inflammatory cytokines concentration for groups treated with dexamethasone and flunixin meglumine (P%26gt;0.05). The findings also revealed that immediate intravenous administration of dexamethasone and flunixin meglumine reduced and controlled the acute phase response in sheep endotoxemia due to Escherichia colt serotype O55:B5.

• 出版日期2013-6