Automated, parallel mass spectrometry imaging and structural identification of lipids

Ellis Shane R; Paine Martin R L; Eijkel Gert B; Pauling Josch K; Husen Peter; Jervelund Mark W; Hermansson Martin; Ejsing Christer S<sup>*</sup>; Heeren Ron M A<sup>*</sup>

doi:10.1038/s41592-018-0010-6

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Automated, parallel mass spectrometry imaging and structural identification of lipids

作者：Ellis Shane R; Paine Martin R L; Eijkel Gert B; Pauling Josch K; Husen Peter; Jervelund Mark W; Hermansson Martin; Ejsing Christer S^*; Heeren Ron M A^*

来源：Nature Methods, 2018, 15(7): 515-+.

DOI：10.1038/s41592-018-0010-6

摘要

We report a method that enables automated data-dependent acquisition of lipid tandem mass spectrometry data in parallel with a high-resolution mass spectrometry imaging experiment. The method does not increase the total image acquisition time and is combined with automatic structural assignments. This lipidome-per-pixel approach automatically identified and validated 104 unique molecular lipids and their spatial locations from rat cerebellar tissue.