科研之友
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摘要
Present study evaluated the anti-clastogenic efficacy of Azadirachta indica (A. indica) against benzo(a)pyrene 113(a)PI in murine forestomach tumorigenesis bioassay protocol. Female Balb/c mice were divided into four groups (n = 8). Each mouse from B(a)P and 13(a)P + A. indica groups received intragastric instillations of B(a)P at a dose of 40 mg/kg b. w. in 0.2 mL olive oil twice a week, starting (roil) 3(rd) week to the end of 6(th) week of the experiment. Mice of control and A. indica groups received 0.2 mL olive oil in the same schedule as or B(a)P and 13(a)P + A indica groups. Mice of A. indica and B(a)P + A. indica groups received oral doses of 100 mg/kg b. w. aqueous A. indica leaf extract (AAILE) on alternate clays throughout the experiment. Two weeks alter the last B(a)P instillation. mice were sacrificed and spleens were processed for micronucleus (MN) assay, while liver tissues were analyzed for lipid peroxidation (LPO), as well as antioxidant defense enzymes, namely: catalase, superoxide dismutase (SOD), glutathione reductase (GR) and glutathione peroxidase (GPx). The incidence of MN formation increased in spleen cells of mice that received only 13(a)P instillations. In hepatic tissues, the extent of oxidative stress increased upon B(a)P instillations as was evidenced from enhanced LPO levels with concomitant decrease in antioxidant defense enzyme activities in mice that received only B(a)P instillations. Interestingly. A. indica treatment significantly reversed these effects as observed in inice receiving AAILE along with B(a)P when compared to only B(a)P receiving mice. Moreover. in only AAILF receiving mice. enhanced antioxidant defense with slightly decreased levels of LPO as well as MN incidences were observed. Observations of the present study suggest that A. indica exert anti-clastogenic effects against B(a)P by modulatins2 oxidative stress and antioxidant defense.
- 出版日期2010-8
