Tong Luo Jiu Nao injection, a traditional Chinese medicinal preparation, inhibits MIP-1β expression in brain microvascular endothelial cells injured by oxygen-glucose deprivation

作者：Wang, Jun; Li, Peng-Tao^*; Du, Huan; Hou, Jin-Cai; Li, Wei-Hong; Pan, Yan-Shu; Chen, Hui-Cong

来源：Journal of Ethnopharmacology, 2012, 141(1): 151-157.

DOI：10.1016/j.jep.2012.02.013

• Brain microvascular endothelial cells
• CCR5
• Ischemia
• Microglia
• MIP-1 beta
• Tong Luo Jiu Nao

Ethnopharmacological relevance: Tong Luo Jiu Nao injection (TLJN), a modern Chinese formula based on Traditional Chinese Medicine theory, has been used to treat ischemic stroke and vascular dementia. TLJN belongs to the ethnopharmacological family of medicines. @@@ Aim of the study: To investigate the protective effect of TLJN on oxygen-glucose deprivation (OGD) induced-injury of brain microvascular endothelial cells (BMECs). @@@ Materials and methods: The model of OGD was established in the primarily cultured BMECs. TLJN was added to the OGD-injured BMECs for 6 h. A series of assays were used to detect the effects of TLJN on: (i) MIP-1 beta content in BMECs conditioned media (CM) by ELISA: (ii) MIP-1 beta protein expression in BMECs by western blotting and immunocytochemistry; (iii) the expression of CCR5, receptor of MIP-1 beta, in BMECs by western blotting; (iv) the proliferative activity of microglial cells via the Cell Counting Kit-8 (CCK-8). @@@ Results: Our results showed that the OGD-injured BMECs presented with large amounts of secretion and expression of MIP-1 beta and up-regulation of CCR5. Also, the CM of OGD-injured BMECs remarkably increased the proliferative activity of microglial cells. The TLJN-treated BMECs exhibited a reduction of MIP-1 beta content in BMECs-CM and a down-regulation of MIP-1 beta and CCR5 expression. In addition, an inhibitory effect of CM of OGD-injured plus TLJN injection-treated BMECs on microglial proliferation was also found. @@@ Conclusion: TLJN reduced the expression of MIP-1 beta and CCR5 in OGD-injured BMECs, and the CM of OGD-injured plus TLJN injection-treated BMECs inhibited the proliferative activity of microglial cells, suggesting the therapeutic potential of TLJN on ischemic cerebral vascular disease.

• 出版日期2012-5-7
• 单位中国中医科学院西苑医院; 北京中医药大学; 天津中医药大学