科研之友
微信
新浪微博
Facebook
分享链接
摘要
Objectives: The aim of this study was to evaluate the effect of grape seed extract (GSE) on enamel caries lesion formation in an in vitro Streptococcus mutans biofilm model. Methods: Enamel fragments were prepared from bovine incisors and divided into six treatment groups (n = 12): inoculated Brain Heart Infusion with 1% sucrose (BHIS), 1 mg/mL GSE, 2 mg/mL GSE, 3 mg/mL GSE, 10 ppm fluoride as NaF, and uninoculated BHIS. For biofilm formation, tooth fragments were incubated anaerobically in polystyrene 6-well tissue culture plates containing BHIS, the respective agents, and S. mutans (1 x 10(5) CFU/mL) for 24 h at 37 degrees C. Culture medium was replaced with fresh BHIS and respective agents daily over a 7-day period. Following caries lesion formation, lesion depth (LD) and relative optical density (ROD) were determined by polarized light microscopy (PLM) and confocal laser scanning microscopy (CLSM), respectively, to evaluate lesion progression. Results: LDs of the 2 mg/mL GSE group (122.86 +/- 13.41 mu m) and the 3 mg/mL GSE group (111.92 +/- 11.39 mu m) were significantly smaller than those of the 1 mg/mL GSE (198.33 +/- 17.70 mu m) and control groups (210.86 +/- 15.50 mu m) (p < 0.05). Compared with the 2 mg/mL and 3 mg/mL groups, the control and 1 mg/mL GSE groups showed significantly lower ROD values when depth was less than 200 mu m, indicating greater mineral loss. Conclusions: Dose-dependent GSE inhibits in vitro enamel caries formation due to its ability to suppress growth of S. mutans and the formation of biofilm. Clinical significance: Grape seed extract may be a novel virulence-targeted natural antimicrobial agent for caries prevention. Published by Elsevier B.V.
- 出版日期2014-8
- 单位中山大学
