UP-REGULATION OF ROS SCAVENGING ENZYMES IN atr ARABIDOPSIS THALIANA MUTANTS WITH COMPROMISED PROGRAMMED CELL DEATH

作者：Edreva Aglika^*; Demirevska Klimentina; Gesheva Emilia; Gechev Tsanko

来源：Comptes Rendus de l Academie Bulgare des Sciences, 2011, 64(8): 1131-1136.

• A. thaliana mutants
• programmed cell death
• paraquat
• superoxide dismutase
• catalase
• glutathione-S-transferase
• glutathione reductase

This work describes the alteration in the activities of a range of ROS-scavenging enzymes and nonenzymatic antioxidants in the oxidative stress-sensitive mutant loh2 and oxidative stress-tolerant mutants atr7 and atr9 exposed to paraquat. The activities of superoxide dismutase, catalase, glutathione reductase and glutathione-S-transferase were assayed, as well as the levels of nonenzymatic antioxidants estimated by the FRAP and DPPH circle tests. Spraying loh2 plants with paraquat lead to formation of bleaching spots and decreased activities of ROS-scavenging enzymes and nonenzymatic antioxidants. In contrast, no cell death symptoms were observed both in atr7 and atr9 while enzyme activities and nonenzymatic antioxidants rose up or remained constant after paraquat exposure.

• 出版日期2011