An anther-specific chalcone synthase-like gene D5 related to rice pollen development

作者:Zheng HH; Qu LJ*; Liu MH; Zhang Y; Shen YP; Wei JM; Pan NS; Gu HY; Chen ZL
来源:Chinese Science Bulletin, 2000, 45(21): 1921-1926.
DOI:10.1007/BF02909679

摘要

It was shown in a previous analysis that D5 gene from rice (Oryza saliva L.) was an anther-specific gene encoding a chalcone synthase-related protein. In this study, D5 gene was found specifically expressed in tapetum cells as well as in the peripheral cells of the vascular bundle of rice anthers by RNA in situ hybridization. In order to study its function, D5 was transformed into rice in both sense and antisense directions driven by a rice Actin I promoter. It has been observed that the pollen grains from the antisense D5 transgenic rice plants are abnormal, indicating that D5 plays a critical role in rice pollen development.