Differential apoptotic response in HPV-infected cancer cells of the uterine cervix after doxorubicin treatment

作者：Suh Dong Soo; Kim Seung Chul; An Won Geun; Lee Chang Hun^*; Choi Kyung Un; Song Jin Mi; Jung Jin Seup; Lee Kyu Sup; Yoon Man Soo

来源：Oncology Reports, 2010, 23(3): 751-756.

DOI：10.3892/or_00000694

• apoptosis
• human papilloma virus
• doxorubicin
• cervical cancer

This study aimed to evaluate differential apoptotic response in uterine cervical cancer cells with and without HPV infection after chemotherapy. CaSki (HPV 16-positive) and C33A (mutant p53 and HPV-negative) cells were used. Cell viability was assessed by trypan blue cell exclusion test. Apoptosis was evaluated by DNA fragmentation analysis and flow cytometric analysis. Differential apoptotic responses Were evaluated using Western blot analysis after chemotherapy. Decreased cell viability and apoptosis were displayed in CaSki and C33A cells after chemotherapy using doxorubicin (DOX). Regarding apoptosis-related molecules, the appearance of cleaved PARP expression was more prominent in CaSki cells. p53 and p21 expression in CaSki cells were increased. On the other hand, JNK expression was different in C33A cells (increased) and CaSki cells (decreased). CaSki cells and C33A cells showed differential apoptotic responses after chemotherapy using DOX. CaSki cells seem to be related with p53/P21 expression, but C33A cells seem to be related with PARP-JNK expression, which is involved in the mitochondrial pathway.

• 出版日期2010-3
• 单位河北医科大学