Antioxidant enzymes and inorganic elements in seeds and leaves of four potential medicinal plants from Pakistan

作者：Shahid M^*; Khan M Masud Ul Haq; Hameed A; Ashraf M; Jamil A

来源：Agrochimica, 2012, 56(6): 281-291.

• Croton tiglium
• Hygrophila auriculata
• Moringa oleifera
• phenolics
• Withania somnifera

Extracts of fresh seeds and leaves of four medicinal plants viz. Hygrophila auriculata, Moringa oleifera, Croton tiglium and Withania somnifera were analysed for some metabolically important enzymes, i.e., proteases (PRT), peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT), total phenolic content (TPC) and some inorganic elements (Zn, Cu, Mo, and Se). The highest specific activities of PRT and SOD were recorded in the seeds of M. oleifera and those of POD in the leaf extract of W. somnifera followed by C. tiglium. However, the highest specific activity of CAT was observed in the leaf extracts of H. auriculata (46.27 units/mg protein) followed by M. oleifera (37.82 units/mg protein). The highest TPC was found in the leaf extracts of H. auriculata (0.68 gallic acid equivalents g/100 g fresh weight). Higher concentrations of Zn were recorded in the leaf and seed samples of all four medicinal plants as compared to the other elements analysed (Cu, Mo, and Se). Statistical analysis showed that the antioxidant and enzymatic activities were significantly (p < 0.05) different among medicinal plant, and leaf and seed extracts as well. It was concluded that tested medicinal plants showed a good comparative profile of antioxidant enzymes and elements that will be useful for further studies.

• 出版日期2012-12