A novel antibacterial peptide active against peach crown gall (Agrobacterium tumefaciens) isolated from cyanide-tolerant actinomycetes G19

作者：Wang Shufang; Ji Jinglin; Ma Huanpu; Liu Zhimin^*

来源：World Journal of Microbiology and Biotechnology, 2015, 31(1): 69-74.

DOI：10.1007/s11274-014-1765-2

• Agrobacterium tumefaciens
• Antagonistic actinomycetes
• Antimicrobial peptide
• Peach crown gall

An antimicrobial peptide was extracted from the antagonistic actinomycetes G19. It was designated as G19-F. By using MALDI-TOF mass spectrometry, the molecular weight of G19-F was determined. The primary structure of the antimicrobial peptide was determined using N-terminal sequencing and mass spectrometry. Results showed that the peptide had eleven amino acids, with the sequence D-V-C-D-G-G-D-G-D-E-D, and a calculated molecular mass of 1,096 Da. G19-F showed antimicrobial activity against peach crown gall caused by Agrobacterium tumefaciens. The antimicrobial peptide maintained its activity after being heated to 100 A degrees C and exhibited stability from pH 4 to 10. Its activity has also remained after ultraviolet irradiation. The mechanism by which G19-F inhibits A. tumefaciens was to increase permeability of the cell membrane and destroy the cell wall structure. Furthermore, as a novel peptide, it has a potential for cure A. tumefaciens infection.

• 出版日期2015-1
• 单位北京农学院