Amperometric glucose biosensor based on immobilization of glucose oxidase on a magnetic glassy carbon electrode modified with a novel magnetic nanocomposite

作者：Baghayeri Mehdi; Veisi Hojat; Ghanei Motlagh Masoud

来源：Sensors and Actuators B: Chemical , 2017, 249: 321-330.

DOI：10.1016/j.snb.2017.04.100

• Diabetes
• Direct electron transfer
• Silver nanoparticles
• Glucose oxidase
• Nanocomposite

We present a method to produce a magnetic glassy carbon electrode (MGCE) modified with multilayer nanocomposite (Ag@MWCNT-IL-Fe3O4) as an immobilization support to promote electron transfer reactions of glucose oxidase (GOx). The nanocomposite verified by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and a vibrating sample magnetometer (VSM). The direct electron transfer (DET) between GOx and Ag@MWCNT-IL-Fe3O4 nanocomposite was investigated using cyclic voltammetry which showed a pair of well-defined redox peaks corresponding to redox active center of GOx. Utilization of constructed electrode for detection of glucose was studied in N-2 and air saturated solutions (pH 7.0). The GOx/Ag@MWCNT-IL-Fe3O4/MGCE showed excellent stability, a detection limit of 2.12 mu M, a linear range between 6 mu M and 2 mM, and a dynamic range up to 2.7 mM at the air saturated solutions. Also, in N-2 saturated solutions (pH 7.0), determination of glucose was carried out by amperometry, and the proposed biosensor showed good reproducibility and stability with a detection limit of 3.8 mu M and a linear range between 10 mu M and 1 mM. Such analytical performances confirm that the fabricated biosensor used in this work has potential to be applied for development of redox enzyme based biosensors.

• 出版日期2017-10