(V600E)Braf induces gastrointestinal crypt senescence and promotes tumour progression through enhanced CpG methylation of p16(INK4a)

作者：Carragher Linda A S; Snell Kimberley R; Giblett Susan M; Aldridge Victoria S S; Patel Bipin; Cook Simon J; Winton Doug J; Marais Richard; Pritchard Catrin A^*

来源：EMBO Molecular Medicine, 2010, 2(11): 458-471.

DOI：10.1002/emmm.201000099

• Braf
• intestine
• mouse model
• senescence
• serrated colorectal cancer

The majority of human colorectal cancers (CRCs) are initiated by mutations arising in the adenomatous polyposis coli (APC) tumour suppressor gene. However, a new class of non-APC mutated CRCs has been defined that have a serrated histopathology and carry the (V600E)BRAF oncogene. Here we have investigated the pathogenesis of serrated CRCs by expressing (V600E)Braf in the proliferative cells of the mouse gastrointestinal tract. We show that the oncogene drives an initial burst of Mek-dependent proliferation, leading to the formation of hyperplastic crypts. This is associated with beta-caten in nuclear localization by a mechanism involving Mapk/Erk kinase (Mek)-dependent, Akt-independent phosphorylation of Gsk3 beta. However, hyperplastic crypts remain dormant for prolonged periods due to the induction of crypt senescence accompanied by upregulation of senescence-associated p-galactosidase and p16(Ink4a). We show that tumour progression is associated with down-regulation of p16(Ink4a) through enhanced CpG methylation of exon land knockout of Cdkn2a confirms this gene is a barrier to tumour progression. Our studies identify (V600E)BRAF as an early genetic driver mutation in serrated CRCs and indicate that, unlike APC-mutated cancers, this subtype arises by the bypassing of a (V600E)Braf driven oncogene-induced senescence programme.

• 出版日期2010-11