An Oxidative Bioconjugation Strategy Targeted to a Genetically Encoded 5-Hydroxytryptophan

作者：Addy Partha Sarathi; Italia James S; Chatterjee Abhishek^*

来源：ChemBioChem, 2018, 19(13): 1375-1378.

DOI：10.1002/cbic.201800111

• bioconjugation
• genetic code expansion
• site-specific protein modification
• unnatural amino acid

Approaches that enable the chemoselective, covalent modification of proteins in a site-specific manner have emerged as a powerful technology for a wide range of applications. The electron-rich unnatural amino acid 5-hydroxytryptophan was recently genetically encoded in both Escherichia coli and eukaryotes, thereby allowing its site-specific incorporation into virtually any recombinant protein. Herein, we report the chemoselective conjugation of various aromatic amines to full-length proteins under mild, oxidative conditions that target this site-specifically incorporated 5-hydroxytryptophan residue.

• 出版日期2018-7-4