Potentiometric bioimaging with a large-scale integration (LSI)-based electrochemical device for detection of enzyme activity

作者：Kanno Yusuke; Ino Kosuke^*; Sakamoto Chika; Inoue Kumi Y; Matsudaira Masahki; Suda Atsushi; Kunikata Ryota; Ishikawa Tomohiro; Abe Hiroya; Shiku Hitoshi; Matsue Tomokazu

来源：Biosensors and Bioelectronics, 2016, 77: 709-714.

DOI：10.1016/j.bios.2015.10.021

• LSI-based electrochemical sensors
• Potentiometric bioimaging
• Chip device
• Bioanalysis
• Embryonic stem cells

This paper describes potentiometric bioimaging for enzyme activity using a large-scale integration (LSI)-based electrochemical device with 400 sensors. Potentiometric detection is useful for bioimaging because redox species are not consumed or produced during the detection process; therefore, there is no effect on cell activity and the detectable signal is sustained. In this study, the potentiometer mode of the LSI-based device was applied for the detection of glucose oxidase (GOx) and alkaline phosphatase (ALP) activity. The enzyme activities were quantitatively detected within the concentration ranges of 25-250 mu g/mL and 0.10-5.0 ng/mL. In addition, GOx activity in hydrogels and the ALP activity of embryoid bodies (EBs) from embryonic stem (ES) cells were successfully imaged based on detection of the open circuit potentials of individual sensors in real time. To the best of our knowledge, this is the first report of potentiometric imaging using LSI-based electrochemical arrays to detect enzyme activity in ES cells. The LSI-based device is thus demonstrated to be a promising tool for bioimaging of enzyme activity.

• 出版日期2016-3-15