科研之友
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摘要
A novel analytical method of nonionic micellar high-performance liquid chromatography has been developed to simultaneously separate and determine three active ingredients in cold compound preparations, in which the contents of paracetamol were significantly higher than that of pseudoephedrine and chlorpheniramine. A C18 column was used as the stationary phase and ultraviolet detection wavelengths were selected at 210nm for chlorpheniramine and pseudoephedrine and 300nm for paracetamol. The micellar mobile phase was composed of an aqueous solution (containing 3.0x10(-3)mol/L Brij-35 and 2.0x10(-2)mol/L potassium dihydrogen phosphate)-methanol (96:4, v/v) at pH 3. Under the conditions, the analytes were separated in 10min. The method was validated by limit of quantitation, limit of detection, linearity, stability, precision, and recovery. The possible separation mechanism of the proposed method was also briefly discussed. Compared with sodium dodecyl sulfate and cetyltrimethylammonium bromide, the ultraviolet absorption produced by Brij-35 can be neglected. The novel method used much lower amount of organic solvent (4%, v/v). Programmed ultraviolet detection improved the accuracy of simultaneous determination of the analytes whose contents were in great disparity. The method was successfully applied to determine pseudoephedrine, paracetamol, and chlorpheniramine in cold compound preparations.
- 出版日期2015-1-20
- 单位兰州大学
