Microspectroscopic Study of Liposome-to-cell Interaction Revealed by Forster Resonance Energy Transfer

作者：Yefimova Svetlana L^*; Kurilchenko Irina Yu; Tkacheva Tatyana N; Kavok Nataliya S; Todor Igor N; Lukianova Nataliya Yu; Chekhun Vasyl F; Malyukin Yuriy V

来源：Journal of Fluorescence, 2014, 24(2): 403-409.

DOI：10.1007/s10895-013-1305-8

• Forster resonance energy transfer
• Fluorescent dyes
• Liposome
• Living cells

We report the Forster resonance energy transfer (FRET)-labeling of liposomal vesicles as an effective approach to study in dynamics the interaction of liposomes with living cells of different types (rat hepatocytes, rat bone marrow, mouse fibroblast-like cells and human breast cancer cells) and cell organelles (hepatocyte nuclei). The in vitro experiments were performed using fluorescent microspectroscopic technique. Two fluorescent dyes (DiO as the energy donor and DiI as an acceptor) were preloaded in lipid bilayers of phosphatidylcholine liposomes that ensures the necessary distance between the dyes for effective FRET. The change in time of the donor and acceptor relative fluorescence intensities was used to visualize and trace the liposome-to-cell interaction. We show that FRET-labeling of liposome vesicles allows one to reveal the differences in efficiency and dynamics of these interactions, which are associated with composition, fluidity, and metabolic activity of cell plasma membranes.

• 出版日期2014-3