Use of Hydrolysates from Yellowfin Tuna (Thunnus albacares) Heads as a Complex Nitrogen Source for Lactic Acid Bacteria

作者：Safari Reza; Motamedzadegan Ali; Ovissipour Mahmoudreza^*; Regenstein Joe Mack; Gildberg A**jorn; Rasco Barbara

来源：Food and Bioprocess Technology, 2012, 5(1): 73-79.

DOI：10.1007/s11947-009-0225-8

• Lactic acid bacteria
• Fish hydrolysates
• Growth media
• Peptones
• Alcalase
• Protamex
• Fish heads

Two different peptones obtained by enzymatic hydrolysis of yellowfin tuna (Thunnus albacares) head waste have been shown to be effective in promoting the growth of lactic acid bacteria (Lactobacillus bulgaricus Persian Type Culture Collection (PTCC) 1332, Lactobacillus acidophilus PTCC 1643, Lactobacillus casei PTCC 1608, Lactobacillus delbrukii PTCC 1333, Lactobacillus plantarum PTCC 1058, Lactococcus lactis PTCC 1336, and Lactobacillus sakei PTCC 1712). Peptones obtained from the enzymatic hydrolysis with Alcalase or Protamex were used instead of the standard peptones used in commercial MRS media. Peptones produced by Alcalase and Protamex had a 34% and 19% degree of hydrolysis, respectively. The results showed that the peptones from Alcalase and Protamex were better at promoting lactic acid bacteria (LAB) growth than the commercial MRS media (P %26lt; 0.05). The choice of proteolytic enzyme used to produce the fish hydrolysate had a considerable impact on the performance of the resulting hydrolysate, both in terms of maximum growth rate and biomass production. Peptones produced using Alcalase, with a higher degree of hydrolysis, induced better growth and performed better overall as an LAB substrate than those using Protamex. Current study revealed that enzymatic-modified fish by-products can be used as low cast nitrogen source for bacterial growth.

• 出版日期2012-1