科研之友
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摘要
The aim of the present study was to perform a comparative phytochemical and pharmacological evaluation of organically and non-organically cultivated nutritional Centella asiatica collected at different time intervals of harvesting. The fertilizers and pesticides applied in case of organic cultivation included combination of medicinal plants along with other natural products and the harvesting was carried out after 1st, 2nd and 3rd month of cultivation. From all the physicochemical parameters evaluated, the leaves collected after third month of harvesting showed higher ash values suggesting higher contents of sand or earthy matter along with higher moister content. The results from the phytochemical evaluations revealed that, the samples collected from first harvesting showed higher quantities of phytoconstituents. This was also confirmed through chromatographic studies using HPTLC showing higher content of Asiatic acid in samples collected after first month of harvesting, which was more pronounced in case of organic cultivation. The pharmacological evaluation included comparative nootropic activity of different samples of organic and nonorganic C. asiatica. The results depicted a significant (p < 0.05) reduction in escape latency of mice treated with 100 mg/kg, p.o. of organic and nonorganic cultivated C. asiatica collected after first month of harvesting and also showed a significant increase in the spatial working memory, which included acquisition and retrieval trials. Further, the two samples also revealed significant inhibition in AChE activity as observed in different brain region i.e. hippocampus, prefrontal cortex and amygdale, where organically cultivated C. asiatica was found to be more effective. Thus, from the overall observation, it may be concluded that, the organically cultivated C. asiatica, if collected after first month of cultivation shows the best memory enhancing activity as compared to other time interval of harvesting.
- 出版日期2017-9
