Differential Pigment Accumulation in Carrot Leaves and Roots during Two Growing Periods

作者：Perrin Florent; Brahem Marwa; Dubois Laurent Cecile; Huet Sebastien; Jourdan Matthieu; Geoffriau Emmanuel; Peltier Didier; Gagne Severine^*

来源：Journal of Agricultural and Food Chemistry, 2016, 64(4): 906-912.

DOI：10.1021/acs.jafc.5b05308

• carotenoid
• chlorophyll
• Daucus carota L.
• environmental factors
• growing conditions
• transcriptional regulation

Carotenoids are important secondary metabolites involved in plant growth and nutritional quality of vegetable crops. These pigments are highly accumulated in carrot root, but knowledge about the impact of environmental factors on their accumulation is limited. The purpose of this work was to investigate the impact of environmental variations on carotenoid accumulation in carrot leaves and roots. In this work, carrots were grown during two contrasting periods to maximize bioclimatic differences. In leaves, carotenoid and chlorophyll contents were lower in the less favorable growing conditions, whereas relative contents were well conserved for all genotypes, suggesting a common regulatory mechanism. The down-regulation of all genes under environmental constraints demonstrates that carotenoid accumulation is regulated at the transcriptional level. In roots, the decrease in alpha-carotene and lutein contents was accompanied by an increase of beta-carotene relative content. At the transcriptional level, LCYB and ZEP expression increased, whereas LCYE expression decreased, in the less favorable conditions, suggesting that carotenoid biosynthesis is switched toward the beta-branch.

• 出版日期2016-2-3