ABC transporter function and regulation at the blood-spinal cord barrier

作者：Campos Christopher R; Schroeter Christian; Wang Xueqian; Miller David S^*

来源：Journal of Cerebral Blood Flow and Metabolism, 2012, 32(8): 1559-1566.

DOI：10.1038/jcbfm.2012.47

• capillaries
• confocal imaging
• Dioxin
• membrane transport
• P-glycoprotein

We present here an initial characterization of ATP binding cassette (ABC) transporter function and regulation at the blood-spinal cord barrier. We isolated capillaries from rat spinal cords and studied transport function using a confocal microscopy-based assay and protein expression using western blots. These capillaries exhibited transport function and protein expression of P-glycoprotein (Abcb1), multidrug resistance protein 2 (Mrp2, Abcc2), and breast cancer-related protein (Bcrp, Abcg2). Exposing isolated capillaries to dioxin (activates aryl hydrocarbon receptor) increased transport mediated by all three transporters. Brain and spinal cord capillaries from dioxin-dosed rats exhibited increased P-glycoprotein-mediated transport and increased protein expression for all three ABC transporters. These findings indicate similar ABC transporter expression, function, and regulation at the blood-spinal cord and blood-brain barriers. Journal of Cerebral Blood Flow & Metabolism (2012) 32, 1559-1566; doi: 10.1038/jcbfm.2012.47; published online 4 April 2012

• 出版日期2012-8
• 单位NIH