Differentiating blood samples from scrapie infected and non-infected hamsters by detecting disease-associated prion proteins using Multimer Detection System

作者：An S S A^*; Lim K T; Oh H J; Lee B S; Zukic E; Ju Y R; Yokoyama T; Kim S Y; Welker E

来源：Biochemical and Biophysical Research Communications, 2010, 392(4): 505-509.

DOI：10.1016/j.bbrc.2010.01.053

• TSE
• Prion
• Blood plasma
• Detection
• ELISA
• Aggregation
• Multimers
• Monomer
• Antibody

This communication describes the application of a modified sandwich enzyme-linked immunosorbent assay (ELISA), termed Multimer Detection System (MDS) for the detection of disease-associated multimeric forms of the prion protein (PrPd) in hamster blood. PrPd was detected in plasma of prion-affected hamsters while MDS revealed no PrPd in identically-treated plasma of healthy animals. This is the first report of a single ELISA- based immune detection of PrPd from blood samples.

• 出版日期2010-2-19