A Plausible Anti-Apoptotic Role of Up-Regulated OCT4B1 in Bladder Tumors

作者：Asadzadeh Jamshid; Asadi Malek Hossein; Shakhssalim Nasser; Rafiee Mahmoud Reza; Kalhor Hamid Reza; Tavallaei Mahmoud; Mowla Seyed Javad^*

来源：Urology Journal, 2012, 9(3): 574-580.

• cancer stem cells
• urinary bladder neoplasms
• apoptosis
• neoplasm invasiveness
• prognosis

Purpose: To investigate and compare the expression of OCT4B1 between tumor and non-tumor bladder tissues. %26lt;br%26gt;Materials and Methods: We investigated the expression of OCT4B1 in 30 tumor and non-tumor surgical specimens of the bladder, using the TaqMan real-time polymerase chain reaction approach and by carefully designing primers and probes specific for the amplificaion of the variant. %26lt;br%26gt;Results: Most tumor and non-tumor samples of the bladder showed OCT4B1 expression, but its expression level was significantly higher in the tumors (P %26lt; .002). Moreover, the up-regulation of OCT4B1 was more significant in high-grade tumors compared to the low-grade ones (P %26lt; .05). We have also employed the RNA interference strategy to evaluate the functional role of OCT4B1 in a bladder cancer cell line, 5637. Suppression of OCT4B1 caused some changes in cell cycle distribution, and significantly elevated the rate of apoptosis in the cells. %26lt;br%26gt;Conclusion: Our findings suggest that OCT4B1 plays a potential role in tumor initiation and/or progression of the bladder cancer. Additionally, OCT4B1 can be regarded as a new tumor marker for detection, classification, and treatment of the bladder cancer. However, more experimental studies are needed to replicate our findings.

• 出版日期2012