A hydrogel-based stem cell delivery system to treat retinal degenerative diseases

作者：Ballios Brian G; Cooke Michael J; van der Kooy Derek; Shoichet Molly S^*

来源：Biomaterials, 2010, 31(9): 2555-2564.

DOI：10.1016/j.biomaterials.2009.12.004

• Hyaluronan
• Hydrogel
• Retina
• Stem cell
• Cell viability
• Cell spreading

Regenerative strategies for retinal degenerative diseases are limited by poor Cellular survival distribution and Integration after transplantation to the sub-retinal space To overcome this limitations a stem cell delivery system was developed, taking advantage of the minimally-invasive, injectable and biodegradable properties of a blend Of hyaluronan and methylcellulose (HAMC) The physical and biological properties of this unique HAMC formulation were studied HAMC supported retinal stern-progenitor cell (RSPC) survival and proliferation in vitro The blend Was a VISCOUS Solution, exhibiting properties ideal for delivery to the sub-retinal space In vivo transplantation Studies in mice were carried out to investigate both the biodegradability of HAMC in the sub-retinal space over 7 clays and the potential of HAMC as a cell delivery vehicle RSPCs delivered in HAMC were more evenly distributed in the sub-retinal Space than those delivered in traditional saline Solutions. Suggesting that HAMC is a promising vehicle for Cellular delivery to th

• 出版日期2010-3
• 单位河北医科大学