Added Value of Next-Generation Sequencing for Multilocus Sequence Typing Analysis of a Pneumocystis jirovecii Pneumonia Outbreak

作者：Charpentier Elena; Garnaud Cecile; Wintenberger Claire; Bailly Sebastien; Murat Jean Benjamin; Rendu John; Pavese Patricia; Drouet Thibault; Augier Caroline; Malvezzi Paolo; Thiebaut Bertrand Anne; Mallaret Marie Reine; Epaulard Olivier; Cornet Muriel; Larrat Sylvie; Maubon Daniele^*

来源：Emerging Infectious Diseases, 2017, 23(8): 1237-1245.

DOI：10.3201/eid2308.161295

Pneumocystis jirovecii is a major threat for immunocompromised patients, and clusters of pneumocystis pneumonia (PCP) have been increasingly described in transplant units during the past decade. Exploring an outbreak transmission network requires complementary spatiotemporal and straintyping approaches. We analyzed a PCP outbreak and demonstrated the added value of next-generation sequencing (NGS) for the multilocus sequence typing (MLST) study of P. jirovecii strains. Thirty-two PCP patients were included. Among the 12 solid organ transplant patients, 5 shared a major and unique genotype that was also found as a minor strain in a sixth patient. A transmission map analysis strengthened the suspicion of nosocomial acquisition of this strain for the 6 patients. NGS-MLST enables accurate determination of subpopulation, which allowed excluding other patients from the transmission network. NGS-MLST genotyping approach was essential to deciphering this outbreak. This innovative approach brings new insights for future epidemiologic studies on this uncultivable opportunistic fungus.

• 出版日期2017-8