Assessment of Reproducibility of Exhaled Hydrogen Peroxide Concentration and the Effect of Breathing Pattern in Healthy Subjects

作者：Gajdocsi Reka; Bikov Andras; Antus Balazs^*; Horvath Ildiko; Barnes Peter J; Kharitonov Sergei A

来源：Journal of Aerosol Medicine and Pulmonary Drug Delivery, 2011, 24(6): 271-275.

DOI：10.1089/jamp.2011.0875

• breathing
• exhaled breath condensate
• hydrogen peroxide
• pattern
• variability

Background: Hydrogen peroxide (H(2)O(2)) is detectable in exhaled breath condensate (EBC) and has been proposed to be a surrogate marker of oxidative stress in the airways. In this study we tested whether the breathing pattern during EBC collection influences the concentration of exhaled H(2)O(2).
Methods: EBC was collected during (1) tidal breathing and (2) breathing with increased tidal volume for 10 min from 16 healthy volunteers. On-line H(2)O(2) measurement was performed by the EcoCheck (TM) biosensor system. Repeated measurements were also conducted to assess intrasubject reproducibility.
Results: Minute ventilation, tidal volume, expiratory flow rate were all increased significantly when subjects were asked to perform breathing with increased tidal volume. In parallel, EBC volume increased (1413 +/- 59 vs. 1959 +/- 71 mu L, p < 0.001), whereas exhaled H(2)O(2) levels decreased significantly (1400 +/- 170 vs. 840 +/- 130 nmol/L, p < 0.001). H(2)O(2) levels did not correlate with any individual breathing parameters (p > 0.05). Assessment of intersubject variability of H(2)O(2) measurements during the two types of breathing revealed a coefficient of variation of 49 and 54%, respectively. The EBC H(2)O(2) measurement was highly reproducible (888 +/- 176 vs. 874 +/- 156 nmol/L) as tested during normal breathing.
Conclusions: These data demonstrate that the concentration of H(2)O(2) in EBC depends on the ventilatory pattern during sample collection that has to be taken into consideration in all EBC H(2)O(2) assays.

• 出版日期2011-12