A noncanonical amino acid-based relay system for site-specific protein labeling

作者：Chen Yuda; Loredo Axel; Gordon Aviva; Tang Juan; Yu Chenfei; Ordonez Janett; Xiao Han^*

来源：Chemical communications, 2018, 54(52): 7187-7190.

DOI：10.1039/c8cc03819h

Genetically site-specific introduction of noncanonical amino acids (ncAAs) for protein conjugation generally requires incorporation through exogenous feeding of chemically synthesized ncAAs. We developed a p-amino-phenylalanine (pAF)-based relay system that enables site-specific functionalization of proteins without chemical synthesis of the building blocks. pAF was biosynthesized under optimized conditions, followed by site-specific incorporation into a specific protein residue. The resulting protein was ready for functionalization using an oxidative conjugation reaction. We demonstrated the use of this relay system by preparing a fluorophore-labeled anti-HER2 single-chain variable fragment antibody for fluorescent imaging.

• 出版日期2018-7-4