Methane oxidation in a landfill cover soil reactor: Changing of kinetic parameters and microorganism community structure

作者：Xing, Zhi L.; Zhao, Tian T.^*; Gao, Yan H.; Yang, Xu; Liu, Shuai; Peng, Xu Y.

来源：Journal of Environmental Science and Health - Part A: Toxic/Hazardous Substances & Environmental Engineering , 2017, 52(3): 254-264.

DOI：10.1080/10934529.2016.1253394

• Biogas
• diffusion
• half-saturation constant
• landfill: methanotrophs
• microbial diversity
• MiSeq sequencing
• model

Changing of CH4 oxidation potential and biological characteristics with CH4 concentration was studied in a landfill cover soil reactor (LCSR). The maximum rate of CH4 oxidation reached 32.40 mol d 1 m 2 by providing sufficient O2 in the LCSR. The kinetic parameters of methane oxidation in landfill cover soil were obtained by fitting substrate diffusion and consumption model based on the concentration profile of CH4 and O2. The values of Km; CH4 (0.93-2.29%) and Vmax; CH4 (140-524 nmol kgsoil-DW 1 s 1) increased with CH4 concentration (9.25-20.30%), while the values of Km; O2 (312.9-2.6%) and Vmax; O2 (1.3 oe 10 5 to 9.0 oe 10 3 nmol mL 1 h 1) were just the opposite. MiSeq pyrosequencing data revealed that Methylobacter (the relative abundance was decreased with height of LCSR) and Methylococcales_ unclassified (the relative abundance was increased expect in H 80) became the key players after incubation with increasing CH4 concentration. These findings provide information for assessing CH4 oxidation potential and changing of biological characteristics in landfill cover soil.

• 出版日期2017
• 单位重庆大学; 重庆理工大学