Medium optimization for phenazine-1-carboxylic acid production by a gacA qscR double mutant of Pseudomonas sp M18 using response surface methodology

作者：Su, Jin-Jiang; Zhou, Quan; Zhang, Hong-Yan; Li, Ya-Qian; Huang, Xian-Qing; Xu, Yu-Quan^*

来源：Bioresource Technology, 2010, 101(11): 4089-4095.

DOI：10.1016/j.biortech.2009.12.143

• Pseudomonas sp M18GQ
• Phenazine-1-carboxylic acid
• Optimizing medium components
• Fractional factorial design
• Response surface methodology

Statistics based experimental designs were applied to optimize the culture medium components for enhancing phenazine-l-carboxylic acid (PCA) production by Pseudomonas sp. strain M18GQ a gacA qscR double mutant of Pseudomonas sp. strain M18. The medium components, including soybean meal, glucose and corn steep liquor, had significant effects on PCA production based on a 2(5-1) fractional factorial design. The concentrations of these three significant factors were subsequently optimized using a central composite design. An optimum concentration of soybean meal 73.3 g/L, corn steep liquor 18.1 g/L, glucose 17.9 g/L, and ethanol 18.0 ml/L was obtained by response surface analysis. The predicted maximum PCA yield was as high as 4011.5 mg/L, and was confirmed by validation experiments. The double mutant M18GQ produced 4032.2 mg/L PCA, which was almost 2 to 3.3-fold that produced by either single mutant M18G and M18Q. The achieved production level is economically useful for industrial application.

• 出版日期2010-6
• 单位上海交通大学