Direct Shoot Organogenesis of Indian Cotton G. Arboreum CV PA 402 from Embryonic Axis Explants

作者：Talegaokar A P^*; Dangat S S

来源：Research Journal of Biotechnology, 2010, 5(3): 73-75.

• Embryonic axis
• organogenesis
• MS media
• regeneration
• kinetin

Cotton is multipurpose crop serving as an engine of economic growth in both developing and developed countries across six continents. India is the only country to grow all four cultivated (both diploid as well as tetraploid) species on commercial scale. The diploid cotton species can serve as valuable gene pool for the agronomically desirable tetraploid cultivars and offer better opportunities. to study gene structure and function through gene knockouts. Development of transgenic lines is one of the ways for value addition in terms of transfer of different agronomic traits as well as study of different gene functions using different tetraploid and diploid germplasm backgrounds. Regeneration protocol through somatic embryo genesis is primary requisite to exploit valuable traits available through plant transformation. In present investigation successful direct shoot organogenesis was attempted in PA 402 variety of diploid cotton using MS media supplemented with Myo-inositol 100 mg/L Thiamine 10 mg/L Glucose 30 gm/L and agar 7 gm/L. The hormone kinetin was used in the range of 0.1 -2.5 mg/L in five different levels. Growth of shoots on MS medium supplemented with Myo-inositol 100 mg/L thiamine 10 mg/L Glucose 30 g/L and NAA 0.1 mg/L resulted in regeneration of plantlets. Among different levels of kinetin 0.1 mg/L was found the most suitable concentration for direct shoot organogenesis from embryonic Axis explants.

• 出版日期2010-8