Steatosis caused by experimental periodontitis is reversible after removal of ligature in rats

作者：dos Santos Carvalho J; Cardoso Guimaraes Vasconcelos A C; Herlany Pereira Alves E; dos Santos Carvalho A; da Silva F R P; de Carvalho Franca L F; de Padua Rocha Nobrega Neto A; Di Lenardo D; de Souza L K M; Barbosa A L dos R; Medeiros J V R; de Oliveira J S; Vasconcelos D F P^*

来源：Journal of Periodontal Research, 2017, 52(5): 883-892.

DOI：10.1111/jre.12459

• histology
• oral medicine
• oxidative stress
• risk factors

Background and ObjectivePeriodontitis may promote harmful systemic effects such as changes in hepatic tissues. The purpose of this study was to investigate whether the steatosis and oxidative stress caused by experimental periodontitis are reversible in the liver.
Material and MethodsTwenty-four rats were divided into three groups: control, periodontitis and P20-20 (20days with experimental periodontitis and 20days without experimental periodontitis, to verify the reversibility of hepatic injuries). The following parameters were assessed: gingival bleeding index, probing pocket depth, myeloperoxidase activity, alveolar bone loss for periodontal tissues; liver weights, histopathological scores for steatosis, inflammation and necrosis in liver; glutathione, malondialdehyde, total cholesterol and triglyceride concentrations in hepatic tissues; and blood levels of aspartate aminotransferase, alanine aminotransferase, albumin, gamma-glutaryl transferase, total cholesterol and random glucose.
ResultsGingival bleeding index, probing pocket depth, myeloperoxidase and alveolar bone loss parameters demonstrated the development of periodontitis. There was a significant reduction in the steatosis score of animals from the P20-20 group when compared with the periodontitis group. P20-20 group presented significantly higher glutathione (11 times) and lower malondialdehyde (nearly 23%), total cholesterol (both in blood and hepatic tissue) and triglyceride concentrations compared with the periodontitis group. For levels of aspartate aminotransferase, alanine aminotransferase, albumin, gamma-glutaryl transferase and random glucose, a significant difference between the groups was not observed.
ConclusionOur results demonstrate that the microvesicular steatosis caused by periodontitis in rats is reversible after removal of the ligature, which is associated with the increase in oxidative stress and lipid peroxidation in the liver.

• 出版日期2017-10