Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends

作者：Zhang, Chunsun; Xing, Da^*

来源：Nucleic Acids Research, 2007, 35(13): 4223-4237.

DOI：10.1093/nar/gkm389

• POLYMERASE-CHAIN-REACTION
• CONTINUOUS-FLOW PCR
• REAL-TIME PCR
• DNA AMPLIFICATION
• REACTION MICROFLUIDICS
• REACTION MICROCHIP
• THROUGH PCR
• DEVICE
• SYSTEM
• TEMPERATURE

The possibility of performing fast and small-volume nucleic acid amplification and analysis on a single chip has attracted great interest. Devices based on this idea, referred to as micro total analysis, microfluidic analysis, or simply 'Lab on a chip' systems, have witnessed steady advances over the last several years. Here, we summarize recent research on chip substrates, surface treatments, PCR reaction volume and speed, architecture, approaches to eliminating cross-contamination and control and measurement of temperature and liquid flow. We also discuss product-detection methods, integration of functional components, biological samples used in PCR chips, potential applications and other practical issues related to implementation of lab-on-a-chip technologies.

• 出版日期2007
• 单位华南师范大学