Assay of bradykinin-related peptides in human body fluids using capillary electrophoresis with laser-induced fluorescence detection

作者：Chen Ying; Xu Liangjun; Lin Jinming; Chen Guionan^*

来源：Electrophoresis, 2008, 29(6): 1302-1307.

DOI：10.1002/elps.200700594

• bradykinin-related peptides
• fluorescein isothiocyanate
• large-volume sample stacking
• laser-induced fluorescence

A CE with LIF detection was developed for separation and determination of bradykinin (BK)-related peptides, such as BK, kallidin (Kal), and neurokinin A (NKA). BK-related peptides were derivatized with FITC prior to CE-LIF analysis. Sodium borate 10 mmol/L at pH 9.5 was selected as derivatization media in order to get the high efficiency. Three peptides were baseline-separated within 10 min by using 110 mmol/L sodium borate-sodium hydroxide solution at pH 10.0 as the running buffer. Concentration detection limits (S/N = 3) for BK, Kal, and NKA were 0.08, 0.5, and 0.2 nmol/L, respectively. Meanwhile we have also developed a simple, quick, and sensitive large-volume sample stacking (LVSS) technique for CE-LIF detection of BK, Kal, and NKA. By using this stacking technique, the detection limits (S/N = 3) for BK, Kal, and NKA were 0.02, 0.05, and 0.04 nmol/L, respectively. This method has been applied to the assay of human saliva and cerebrospinal fluid with satisfactory results.

• 出版日期2008-3
• 单位福州大学