Aggregation and conformational changes of bovine beta-lactoglobulin subjected to dynamic high-pressure microfluidization in relation to antigenicity

作者：Zhong, J. Z.; Liu, W.; Liu, C. M.^*; Wang, Q. H.; Li, T.; Tu, Z. C.; Luo, S. J.; Cai, X. F.; Xu, Y. J.

来源：Journal of Dairy Science, 2012, 95(8): 4237-4245.

DOI：10.3168/jds.2012-5333

• beta-lactoglobulin
• antigenicity
• dynamic high-pressure microfluidization (DHPM)
• protein conformation

Our previous research indicated that dynamic high-pressure microfluidization (DHPM) had a significant effect on the antigenicity of beta-lactoglobulin (beta-LG). In this study, aggregation and conformational changes subjected to DHPM (0.1-160 MPa) were investigated in relation to antigenicity. When DHPM pressure increased from 0.1 to 80 MPa, disaggregation of beta-LG samples and partial unfolding of the molecule were accompanied by an increase in beta-LG antigenicity, which was reflected in the decrease of particle size, increase of free sulfhydryl (SH) contents and beta-strands contents, and slight exposure of aromatic amino acid residues. At pressures above 80 MPa, the reaggregation of beta-LG may contribute to the decrease in antigenicity, which was reflected by an increase in particle size, the formation of aggregates, a decrease of in SH and beta-strands contents, and slight changes in aromatic amino acid residues. Aggregation and conformational changes of beta-LG under DHPM was related to its antigenicity.

• 出版日期2012-8
• 单位中国热带农业科学院; 食品科学与技术国家重点实验室; 南昌大学