Association of miR-146aC > G, miR-196a2T > C, and miR-499A > G Polymorphisms With Risk of Premature Ovarian Failure in Korean Women

作者：Rah HyungChul; Jeon Young Joo; Shim Sung Han; Cha Sun Hee; Choi Dong Hee; Kwon Hwang; Kim Ji Hyang; Shin Ji Eun; Kim Nam Keun^*

来源：Reproductive Sciences, 2013, 20(1): 60-68.

DOI：10.1177/1933719112450341

• premature ovarian failure
• primary ovarian insufficiency
• microRNA
• single nucleotide polymorphism

We investigated whether microRNA (miRNA) polymorphisms (miR-146aC > G, miR-196a2T > C, and miR-499A > G) confer risk of premature ovarian failure (POF) in Korean women. DNA samples from 136 patients with POF and 234 controls were genotyped for the 3 miRNA single-nucleotide polymorphisms by polymerase chain reaction-restriction fragment length polymorphism. The miR-146aCG/miR-196a2TC combined genotype was less frequent in patients than in controls (P < .05), conferring less susceptibility. Using haplotype-based multifactor dimensionality reduction analysis, the C-C-A and G-T-A inferred haplotypes (miR-146a/miR-196a2/miR-499) were less frequent in patients, suggesting protective effects (P < .05 for each), whereas the C-T-A and G-C-A haplotypes were more frequent in patients (P < .05 for each). The C-T and G-C haplotypes (miR-146a/miR-196a2) were more frequent in patients, whereas the C-C and G-T haplotypes were less frequent in patients (P < .05 for each). However, none of the 3 miRNA polymorphisms alone was associated with POF risk. Our findings suggest that putative gene-gene interaction between miR-146 and miR-196a2 may be involved in POF development.

• 出版日期2013-1