Assessing Heterogeneity of Osteolytic Lesions in Multiple Myeloma by H-1 HR-MAS NMR Metabolomics

作者：Tavel Laurette; Fontana Francesca; Manteiga Jose Manuel Garcia; Mari Silvia; Mariani Elisabetta; Caneva Enrico; Sitia Roberto; Camnasio Francesco; Marcatti Magda; Cenci Simone^*; Musco Giovanna^*

来源：International Journal of Molecular Sciences, 2016, 17(11): 1814.

DOI：10.3390/ijms17111814

• high-resolution magic-angle spinning (HR-MAS)
• metabolomics
• multiple myeloma
• nuclear magnetic resonance (NMR)
• osteolysis

Multiple myeloma (MM) is a malignancy of plasma cells characterized by multifocal osteolytic bone lesions. Macroscopic and genetic heterogeneity has been documented within MM lesions. Understanding the bases of such heterogeneity may unveil relevant features of MM pathobiology. To this aim, we deployed unbiased H-1 high-resolution magic-angle spinning (HR-MAS) nuclear magnetic resonance (NMR) metabolomics to analyze multiple biopsy specimens of osteolytic lesions from one case of pathological fracture caused by MM. Multivariate analyses on normalized metabolite peak integrals allowed clusterization of samples in accordance with a posteriori histological findings. We investigated the relationship between morphological and NMR features by merging morphological data and metabolite profiling into a single correlation matrix. Data-merging addressed tissue heterogeneity, and greatly facilitated the mapping of lesions and nearby healthy tissues. Our proof-of-principle study reveals integrated metabolomics and histomorphology as a promising approach for the targeted study of osteolytic lesions.

• 出版日期2016-11