Direct chiral resolution of malic acid in apple juice by ligand-exchange capillary electrophoresis using copper(II)-L-tartaric acid as a chiral selector

作者：Kodama S^*; Yamamoto A; Matsunaga A; Soga T; Hayakawa K

来源：Electrophoresis, 2001, 22(15): 3286-3290.

DOI：10.1002/1522-2683(200109)22:15<3286::AID-ELPS3286>3.0.CO;2-J

• ligand exchange
• capillary electrophoresis
• malic acid
• chiral resolution
• apple juice

Chiral resolution of native DL-malic acid was achieved by ligand-exchange capillary electrophoresis using copper(II)-L-tartrate as a chiral selector. Factors affecting chiral resolution, migration time, and peak area of malic acid were studied. The running conditions for optimum separation of malic acid were found to be 1 mm copper(II) sulfate-1 MM L-tartrate (pH 5.1) with an effective voltage of -20 kV at 30 degreesC, using direct detection at 280 nm, and resolution (R-s) of racemic malic acid was approximately 4. With this system, D- and L-malic acids in apple juice were analyzed successfully.

• 出版日期2001-9