广东地区副猪嗜血杆菌的耐药表型和基因及其进化分析

作者：Wan Xiu-Lin; Li Chun-Ling^*; Liu Zong-Bao; Wang Guo-Xia; Yan He^*

来源：Modern Food Science and Technology, 2020, 36(8): 23-30.

DOI：10.13982/j.mfst.1673-9078.2020.8.1021

• Drug resistance gene
• Drug sensitivity test
• Evolutionary analysis
• Glaesserella parasuis
• Whole genome sequencing

In this study, the genetic relationship, drug sensitivity, and drug resistance genes carried by Glaesserella parasuis in Guangdong province were investigated. The evolution of 16S rRNA of the 53 Glaesserella parasuis strains isolated from different regions in Guangdong was analysed, and their susceptibility to 21 antibacterial drugs was analyzed using the K-B disc diffusion method and the carried drug resistance genes were analyzed using the whole genome sequencing (WGS). Results showed that the strains had high drug resistance rates to oxacillin, trimethoprim-sulfamet, streptomycin and tetracycline, and the multi-drug resistant strains accounted for a relatively high rate (84.91%). A comparison with the drug resistance gene database revealed 21 drug resistance genes that mediate resistance to 9 major classes of antibiotics, among which kasugamycin-resistant gene, ksgA, and bacitracin-resistant gene, bacA, were detected for the first time. The coincidence rate between the aminoglycoside and lincomycin resistance genes and the resistance phenotypes reached 100%. The 16S rRNA gene analysis a close genetic relationship between the Glaesserella parasuis strains from different regions, and a correlation between to the carried drug-resistant genes and the 16S rRNA evolution and isolation time. The results of this study can be used to guide the prevention and treatment of Glaesserella parasuis diseases in Guangdong. ? 2020, Editorial Board of Modern Food Science and Technology. All right reserved.

• 出版日期2020
• 单位华南理工大学