Porcine milk-derived exosomes promote proliferation of intestinal epithelial cells

作者：Chen, Ting; Xie, Mei-Ying; Sun, Jia-Jie; Ye, Rui-Song; Cheng, Xiao; Sun, Rui-Ping; Wei, Li-Min; Li, Meng; Lin, De-Lin; Jiang, Qing-Yan; Xi, Qian-Yun^*; Zhang, Yong-Liang^*

来源：Scientific Reports, 2016, 6(1): 33862.

DOI：10.1038/srep33862

• BREAST-MILK
• GASTROINTESTINAL-TRACT
• MESSENGER-RNA
• IN-VITRO
• EXPRESSION
• P53
• MICRORNAS
• ACTIVATION
• KINASES
• PATTERN

Milk-derived exosomes were identified as a novel mechanism of mother-to-child transmission of regulatory molecules, but their functions in intestinal tissues of neonates are not well-studied. Here, we characterized potential roles of porcine milk-derived exosomes in the intestinal tract. In vitro, treatment with milk-derived exosomes (27 +/- 3 ng and 55 +/- 5 ng total RNA) significantly promoted IPEC-J2 cell proliferation by MTT, CCK8, EdU fluorescence and EdU flow cytometry assays. The qRT-PCR and Western blot analyses indicated milk-derived exosomes (0.27 +/- 0.03 mu g total RNA) significantly promoted expression of CDX2, IGF-1R and PCNA, and inhibited p53 gene expression involved in intestinal proliferation. Additionally, six detected miRNAs were significantly increased in IPEC-J2 cell, while FAS and SERPINE were significantly down-regulated relative to that in control. In vivo, treated groups (0.125 mu g and 0.25 mu g total RNA) significantly raised mice' villus height, crypt depth and ratio of villus length to crypt depth of intestinal tissues, significantly increased CDX2, PCNA and IGF-1R' expression and significantly inhibited p53' expression. Our study demonstrated that milk-derived exosomes can facilitate intestinal cell proliferation and intestinal tract development, thus giving a new insight for milk nutrition and newborn development and health.

• 出版日期2016-9-20
• 单位华南热带农业大学; 华南农业大学